Separation of enantiomers of chiral beta-blocker drugs on polysaccharide-based chiral columns and aqueous mobile phases in high-performance liquid chromatography

In the present study the separation of chiral -blockers on polysaccharide-based chiral columns and aqueous mobile phase was performed. Decrease of retention times was observed with increasing water content in acetonitrile on all studied chiral columns. This indicates that commonly accepted reversed-phase mechanism is not involved in separation of enantiomers in this particular separation system and the separation follows to hydrophilic-interaction chromatographic separation (HILIC) mechanism.

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